Fakultät für Chemie - Former
 
 
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Bielefeld University > Department of Chemistry > Organic Chemistry III > Team > Former

Personal data for Dr. Carolin Plattner (née Heggemann)

  Dr. Carolin Plattner (née Heggemann)
Dr. Carolin Plattner (née Heggemann)
Scientific co-worker
Room: F2-231
Phone: 0521 106 2152
E-Mail:


Research



Illustration 1: Primary structure of AFGP.


Biological antifreezes enable life in subpolar seas at water temperatures as low as 1.9 °C. There are four classes of structural diverse antifreeze proteins (AFPI-IV) and a single class of antifreeze glycoproteins (AFGP), the latter being further divided into eight subtypes according to the covered mass fraction. In case of AFGP, little is known about the molecular mechanism of antifreeze activity due to the tedious purification from natural sources and challenging synthesis of heavily glycosylated peptides. All AFGP consist of a varying number of –(Ala-Ala-Thr)n (n=4-50) with every threonine side chain being glycosylated with β-D-galactosyl-(1-3)-α-N-acetyl-D-galactosamine. This pattern is highly conserved among different species. Minor sequence variations where alanine is substituted by proline or the glycosylated threonine by arginine are biologically active. The proteins and peptides initiate thermal hysteresis, change the crystal habit and suppress recrystallization and heterogeneous ice nucleation. Although essential moieties of the peptides are known, the protein/peptide-ice interface is not yet understood. Particularly, the account of sequence variations has barely been elucidated.


Solid phase peptide synthesis enables the preparation of AFGP analogs containing proline and other structure inducing amino acids for eluciation of structure activity relationships. Using the glycosylated amino acid as building block in microwave-enhanced solid phase peptide synthesis, synthetic AFGP analogs are prepared. The peptides are structurally analyzed by NMR and CD. Furthermore, their activity is tested by in recrystallization assays.



The project is supported by the DFG (SFB 613).



Diploma thesis


Synthese von Antigefrierglycopeptiden, Universität Bielefeld, 2004.



Publications


C. Heggemann, C. Budke, B. Schomburg, Z. Majer, M. Wißbrock, T. Koop, N. Sewald, AminoAcids2009, in press.


C. Budke, C. Heggemann, M. Koch, N. Sewald, T. Koop, J. Phys. Chem. B2009, 113, 2865-2873.


A. S. Norgren, C. Budke, Z. Majer, C. Heggemann, T. Koop, N. Sewald, Synthesis2009, 3, 488-494.